Manuel B. Garcia

Manuel B. Garcia serves as the Senior Director for Educational Technology and Digital Learning at FEU Institute of Technology, Manila, Philippines. Read More

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Can a Study Have More Than One Unit of Analysis?

A study can legitimately contain more than one unit of analysis when its research questions operate at different levels or involve relationships across levels. The key is to define each unit explicitly rather than combining them as though all observations represented the same kind of case.

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Multiple Units of Analysis Guide 118 of 223
01 · The Question

Does Every Study Need One and Only One Unit of Analysis?

Suppose you are studying generative AI adoption across universities.

You want to know whether faculty members with greater AI self-efficacy use AI more often. That is an individual-level question.

You also want to know whether universities with stronger AI governance policies have higher institutional adoption rates. That is an organizational-level question.

Finally, you want to know whether university-level policy changes the relationship between individual self-efficacy and faculty adoption. That question crosses levels.

Do you have to choose whether your study is “about faculty” or “about universities”?

Not necessarily.

A study can include more than one unit of analysis when its research questions genuinely operate at multiple levels. What matters is that the study identifies those units explicitly, distinguishes variables and relationships at each level, and uses an analytical approach that respects the resulting structure.

The problem is not having multiple units. The problem is mixing them without realizing it.

02 · The Short Answer

Yes, but Each Unit Must Have a Clear Role

In Brief

A study can have more than one unit of analysis when it asks separate or connected questions about entities at different levels, such as individuals, teams, organizations, or repeated observations within individuals.

Multiple units should not be collapsed into one undifferentiated sample. Researchers need to specify which research questions apply to which units, identify how the units are related or nested, distinguish within-level from cross-level relationships, and use analyses appropriate to the dependence and variation present at each level.

03 · What You Need to Know

Multiple Units Are Legitimate When the Research Problem Is Genuinely Multilevel

Start with the simplest case: one unit of analysis

Many studies focus on one type of entity.

For example:

Students: Is academic self-efficacy associated with persistence?

Schools: Are school resources associated with graduation rates?

Countries: Is national research investment associated with scientific output?

Articles: Is international collaboration associated with citation impact?

In these studies, one main unit can adequately describe the substantive question.

Understanding what the unit of analysis is remains the first step before deciding whether the study actually needs more than one.

Multiple units arise when the questions refer to different kinds of entities

Consider a study involving faculty and universities.

Question 1:

Do faculty members with greater AI self-efficacy report greater AI adoption?

Unit of analysis: faculty member.

Question 2:

Do universities with formal AI governance frameworks have higher institutional adoption rates?

Unit of analysis: university.

The study now contains at least two meaningful analytical units because it asks distinct substantive questions about individuals and organizations.

Multiple units by design Different research questions deliberately concern different entities.
Unit confusion The analysis unintentionally mixes entities without specifying which claims belong to which level.

Having several data sources does not automatically mean having several units of analysis

Suppose a university case study uses faculty interviews, administrator interviews, policy documents, meeting minutes, and website materials.

That is multiple data sources.

But if all sources are integrated to understand one university as a case, the university may remain the single primary unit of analysis.

Participants, documents, and observations provide evidence about that analytical unit.

This is why participants are not automatically units of analysis.

Likewise, many observations do not automatically create many kinds of units

If 500 students each provide one survey response and the study examines individual-level relationships, there may still be only one unit of analysis: student.

If those students are nested within 20 classrooms but classroom differences are irrelevant to the substantive questions, the classrooms are nevertheless part of the data structure, though they may not be substantive analytical targets.

The distinction is important: hierarchy in the data does not automatically mean every level is a substantive unit of analysis.

Nested data frequently create the possibility of multiple analytical levels

Common hierarchies include repeated observations within people, students within classrooms, classrooms within schools, employees within departments, departments within organizations, and patients within hospitals.

These structures are examples of nested or hierarchical data.

The researcher may ask questions at one level, multiple levels, or across levels.

Multiple levels of data are not identical to multiple units of substantive inference

Suppose students are nested within schools.

The outcome is student achievement, and the question asks whether student study habits predict achievement.

School clustering may need to be accounted for statistically because students within the same school are not completely independent.

But the substantive unit of analysis may still be the student.

By contrast, if the study also asks whether school-level leadership explains differences among schools, schools become an explicit analytical level in the scientific argument.

Different levels can contain different variables

Individual-level variables Organization-level variables
Employee self-efficacy Organizational size
Job satisfaction Formal AI policy
Age Leadership structure
AI adoption behavior Technology infrastructure

A multilevel study can examine relationships among individual variables, relationships among organization variables, and relationships connecting the two levels.

This is why distinguishing individual-level and group-level variables becomes important once multiple analytical units are involved.

Within-individual and between-individual questions can also represent multiple levels

Suppose participants report stress and sleep every day.

Researchers could ask whether generally more stressed people sleep less than generally less stressed people. That is a between-person question.

They could also ask whether, on days when a person is more stressed than usual, that person sleeps less than usual. That is a within-person question.

The observations are measurement occasions nested within people. The same variables appear in both questions, but the source of variation differs.

A cross-level relationship connects different analytical levels

Suppose:

University AI policy → Faculty AI adoption

The predictor belongs to the university level. The outcome belongs to the faculty level.

The relationship therefore crosses levels.

Recognizing cross-level relationships prevents researchers from treating variables defined at different levels as ordinary interchangeable columns.

Cross-level moderation adds another layer

Suppose the relationship between individual AI self-efficacy and AI adoption depends on university support.

The proposed relationship is:

Faculty self-efficacy → Faculty adoption

but its strength varies according to university-level support.

This is a cross-level interaction.

Aggregating individuals can create a higher-level analytical unit

Suppose 40 employees within each organization rate organizational climate.

If the theoretical construct is organizational-level and aggregation is justified, researchers may calculate an organization-level score.

Simple Aggregation
Group mean = Σ individual scores / n
Individual observations are combined to produce one value representing a higher-level analytical entity.
If 40 employees' climate ratings sum to 164, the organization mean is 164 / 40 = 4.10. The resulting score may be used as an organization-level variable if the conceptual and measurement assumptions justify aggregation.

This is the logic behind aggregating individual-level data to the group level.

Aggregation does not eliminate the individual level from reality

If individual scores are averaged, variation among individuals within each group disappears from the aggregated dataset.

That may be appropriate for a purely group-level question.

But if researchers also care about individual-level relationships, collapsing everything to group means throws away information needed to answer those questions.

Multilevel models are often designed precisely for this structure

Multilevel, hierarchical, or mixed-effects models allow researchers to represent observations clustered within higher-level units.

A simplified two-level model might distinguish:

Level 1: faculty members

Level 2: universities

Individual outcomes can then be modeled using both faculty-level and university-level characteristics while accounting for dependence among faculty from the same university.

Multiple units require enough information at each relevant level

Suppose 2,000 faculty members are sampled from only eight universities.

The study has extensive individual-level information but very limited between-university information.

Estimating complex university-level effects or cross-level interactions may therefore be difficult even with a large overall participant count.

Watch Out

Lower-level sample size cannot fully compensate for a very small number of higher-level units. If the study asks university-level questions, the number and diversity of universities matter independently of how many faculty members are observed within each university.

The unit relevant to each parameter matters for precision

An individual-level coefficient draws heavily on variation among individuals. A university-level coefficient depends on variation among universities. A cross-level interaction requires information about both.

One headline sample size therefore does not describe the information available for every part of a multilevel study.

Separate analyses at each level can sometimes be appropriate

Not every multiple-unit study requires one enormous multilevel model.

A project might deliberately conduct one faculty-level analysis, one university-level analysis, and one qualitative institution-level comparison.

If these address distinct research questions, separate analyses may be clearer than forcing all questions into one model.

But separate analyses cannot answer every cross-level question

Suppose researchers separately find that faculty self-efficacy predicts individual adoption and that university support predicts institutional adoption rate.

Those two findings do not establish that university support changes the individual self-efficacy–adoption relationship.

That cross-level moderation question requires an analysis capable of linking the levels appropriately.

The same construct can exist at more than one level

Consider support.

At the individual level, perceived personal support may describe one employee's experience.

At the group level, support climate may represent a shared property of an organization.

These constructs are related but should not automatically be treated as identical simply because both use the word “support.”

Group means can separate within-group and between-group information

Suppose individual workload predicts burnout and employees are nested within organizations.

An employee's workload score contains at least two kinds of information: how overloaded that employee is relative to colleagues in the same organization, and whether the organization itself tends to have high average workload.

These can have different relationships with burnout.

A multilevel approach can separate within-group and between-group components rather than assuming one coefficient captures both.

Relationships can differ depending on the unit being compared

Suppose individual employees with greater autonomy report greater satisfaction.

That does not guarantee that organizations with greater average autonomy have greater average satisfaction by exactly the same amount.

The individual-level and organization-level relationships can differ because they represent different sources of variation.

This is why a relationship at one level can differ from the relationship at another.

Multiple units help avoid ecological and atomistic reasoning

If researchers observe a relationship among organizations and assume the same relationship holds among individuals, they risk an ecological fallacy.

If they observe an individual-level relationship and automatically infer an organization-level relationship, they risk an atomistic fallacy.

A multilevel framework can preserve both forms of variation and make clear which conclusion belongs to which analytical unit.

Multiple units should be visible in the research questions

A study claiming to examine multiple levels should not hide that structure until the methods section.

Compare:

RQ1: How is faculty AI self-efficacy related to individual classroom AI adoption?

RQ2: How is university AI infrastructure related to institutional adoption rates?

RQ3: Does university AI infrastructure moderate the relationship between faculty self-efficacy and individual adoption?

The unit and level of each question are visible immediately.

This clarity helps prevent what happens when a research question mixes individual-level and group-level explanations without specifying the intended structure.

Sampling should reflect every substantive unit

If universities are an important analytical unit, the study needs a defensible sample of universities.

If departments are also substantively important, the sampling strategy should provide meaningful departmental variation.

Recruiting hundreds of participants from one or two higher-level units cannot provide broad evidence about variation among higher-level entities.

Do not call every layer a unit of analysis merely because it exists

A dataset may contain items within scales within respondents within teams within organizations.

Not every layer is necessarily a substantive unit of analysis.

Questionnaire items may be measurement indicators rather than substantive analytical entities. Teams may be clustering units without being objects of substantive inference.

Use the term unit of analysis for entities relevant to the actual analytical or inferential question rather than every structural component in the data file.

04 · A Practical Example

One Study, Two Units, and a Cross-Level Question

Hypothetical Example

Faculty members nested within universities

Researchers survey 3,000 faculty members across 60 universities. They measure individual AI teaching self-efficacy and individual AI adoption. They also collect university-level information about AI infrastructure and formal institutional policy.

Individual-level analysis The researchers examine whether faculty AI self-efficacy is associated with individual classroom AI adoption. Unit of analysis: faculty member.
University-level analysis They examine whether universities with stronger AI infrastructure have higher average adoption rates. Unit of analysis: university.
Cross-level analysis They test whether university infrastructure predicts faculty adoption after accounting for individual characteristics.
Cross-level moderation They examine whether the self-efficacy–adoption relationship is stronger in universities with better infrastructure.
Interpretation The project has multiple analytical levels because its questions concern both individual faculty members and universities and explicitly connect those levels.

The overall participant count is 3,000, but the university-level sample contains 60 universities. Each component of the analysis therefore draws on a different source of variation.

05 · What Researchers Often Get Wrong

Common Mistakes When a Study Contains Multiple Units

Misconception

A study is allowed to have only one unit of analysis

No. Multilevel and hierarchical research routinely addresses questions involving more than one unit or level. The requirement is clarity about which question and inference belongs to which unit.

Misconception

If the data contain students and schools, both must be units of analysis

Not necessarily. Schools may simply define clusters while the substantive question concerns students. A structural level becomes a substantive analytical unit only when the study also makes claims about that entity or its variation.

Misconception

Having several data sources means having several units of analysis

No. Interviews, documents, surveys, and observations may all provide evidence about the same organization or case. Data source and analytical unit are separate concepts.

Misconception

Thousands of individual participants provide thousands of group-level cases

No. If 3,000 participants come from 30 universities, there are still only 30 observed universities for university-level comparisons.

Misconception

You can combine variables from different levels without thinking about the hierarchy

Doing so can ignore dependence among lower-level observations and blur within-group, between-group, and cross-level relationships. The analysis should correspond to the data structure and research question.

Misconception

An individual-level relationship must have the same meaning at the group level

No. Relationships based on variation among individuals can differ from relationships based on variation among groups. Moving between levels without evidence risks ecological or atomistic inference errors.

06 · What This Means for You

Use More Than One Unit Only When the Research Questions Require It

A simple decision framework

If every primary question concerns the same type of entity
A single unit of analysis may be sufficient even if the data are clustered.
If separate questions concern individuals and groups
Define both units explicitly and identify which variables and hypotheses belong to each.
If the question links a group-level variable to an individual-level outcome
Treat it as a cross-level relationship and preserve the hierarchical structure analytically.
If individuals provide information used to construct a group-level variable
Justify the aggregation and distinguish the observational source from the resulting analytical unit.
If adding another unit does not answer a distinct theoretical or substantive question
Do not create unnecessary multilevel complexity merely because another structural level exists in the dataset.

A useful planning table can list each research question, focal variables, variable levels, unit of analysis, available number of units at that level, and intended analytical method.

07 · A Quick Checklist

Before Designing a Study With Multiple Units of Analysis, Check This

For every proposed analytical unit, check:
Which research question explicitly concerns this entity?
What variables are defined at this unit or level?
Are lower-level observations nested within higher-level units?
Are any relationships explicitly cross-level?
Is aggregation required, and if so, is it theoretically and empirically justified?
Do I have enough independently observed units at each level for the intended inference?
Does the statistical analysis account for dependence among observations within higher-level units?
Have I separated individual-level, group-level, and cross-level interpretations?
Does every claimed unit contribute to an actual scientific question rather than merely existing in the data structure?
08 · Frequently Asked Questions

Frequently Asked Questions About Multiple Units of Analysis

Can a research study really have more than one unit of analysis?

Yes. A study may examine individuals, groups, organizations, or repeated observations within the same overall project when different research questions operate at those different levels.

Does having nested data automatically mean I have multiple units of analysis?

Not necessarily. The higher-level units may simply create clustering that must be modeled statistically. They become substantive analytical units when the study also asks questions or makes conclusions about variation at that level.

Can individuals and organizations both be units of analysis?

Yes. For example, a study might examine individual employee attitudes and organization-level policies separately and then investigate how organizational characteristics relate to individual outcomes.

Do I need multilevel modeling if I have more than one unit?

Not in every design. Separate analyses may be appropriate for distinct questions, and qualitative case designs may use other approaches. When lower-level observations are clustered and the research question explicitly links levels, multilevel modeling is often useful.

Can I simply average individual data to analyze groups?

Sometimes, but aggregation requires conceptual and measurement justification. Averaging can also remove within-group variation, so it may be unsuitable when individual-level relationships remain part of the question.

If I have 1,000 students from 20 schools, how many units do I have?

You have 1,000 observed students nested within 20 observed schools. Which count matters depends on the question. Student-level relationships use variation among students, whereas school-level relationships depend substantially on variation across the 20 schools.

Can the same variable be analyzed at individual and group levels?

Yes, but the meaning should be explicit. An individual's score and a group mean constructed from those scores represent different sources of variation and can have different relationships with an outcome.

Is a multilevel study automatically better than a single-level study?

No. Multiple levels are useful when the research question genuinely requires them. Adding unnecessary levels increases sampling, measurement, and analytical demands without necessarily improving the scientific answer.

09 · The Bottom Line

A Study Can Have Multiple Units, but the Structure Must Follow the Questions

The Bottom Line

A study can legitimately have more than one unit of analysis when its research questions concern different entities or connect variables across levels, such as individuals within organizations or repeated observations within people.

Do not treat multiple units as one undifferentiated sample. Define which questions, variables, and conclusions belong to each level, ensure adequate information exists at every substantive unit, and use an analytical strategy that respects nesting, dependence, aggregation, and cross-level relationships.

10 · Sources and Further Reading

Sources and Further Reading

11 · Cite this Guide

How to Cite This Guide

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